Downregulated PDIA3P1 lncRNA Impairs Trophoblast Phenotype by Regulating Snail and SFRP1 in PE

Figure 3

PDIA3P1 in trophoblast migration and invasion. (a) Transwell assay assessed the effects of siPDIA3P1 on HTR-8/SVneo and JAR cell migration, with quantification on the right. (b) Transwell assay evaluated the impact of siPDIA3P1 on HTR-8/SVneo and JAR cell invasion, with quantification on the right. (c and d) Cell migration (c) and invasion (d) were confirmed in HTR-8/SVneo and JAR cells transfected with a PDIA3P1-overexpressing plasmid. Quantification is provided below. PDIA3P1 expression was silenced or overexpressed in HUVECs. Performing network formation, cells transfected with siRNAs/overexpressed plasmid targeting PDIA3P1 showed a decrease/increase in node numbers as compared to the negative control. (e) Tube formation images of HUVEC cells transfected with siPDIA3P1 or PDIA3P1 plasmid were photographed. The number of branches (f) and total branch length (g) was analyzed through tube formation assays. Data are reported as mean ± SD. ; .

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