Research Article
Downregulated PDIA3P1 lncRNA Impairs Trophoblast Phenotype by Regulating Snail and SFRP1 in PE
Figure 6
PDIA3P1′s involvement in trophoblast functions via HuR and Snail regulation. FISH analysis of PDIA3P1 distribution in HTR-8/SVneo (a) and JAR cells (b), indicating cytoplasmic localization. (c) Nucleocytoplasmic separation assay confirming subcellular localization of PDIA3P1 in HTR-8/SVneo and JAR cells. (d) RNA pull-down assay demonstrating the direct interaction between HuR and PDIA3P1 in HTR-8/SVneo cells. (e) RNA immunoprecipitation (RIP) experiments evaluating associations between PDIA3P1-HuR and HuR-Snail in HTR-8/SVneo cells. (f) Western blot analysis revealed changes in Snail, E-cadherin, and N-cadherin expression after PDIA3P1 overexpression in HTR-8/SVneo and JAR cells. Data are presented as mean ± SD. ; .
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