Living at the Limits: Evidence for Microbial Eukaryotes Thriving under Pressure in Deep Anoxic, Hypersaline Habitats

Figure 2

Partitioning of protistan phylotypes (called at 98% sequence similarity) between the compared 18S rDNA clone libraries from the interfaces (I) and brines (B) of the L’Atalante (A) [18], Bannock (B) [19], Discovery (D) [19], and Thetis (T) [20] DHABs. Corresponding sequences were retrieved from GenBank’s nucleotide database based on the accession numbers given in the aforementioned publications. Sequences were then clustered in QIIME [95] at 98% sequence similarity before conducting a network analysis in QIIME. For visualization of the network we used Cytoscape [96]. Taxonomic assignment of sequences was conducted with JAguc [97].