Emerging Challenges in Staphylococcus aureus Bloodstream Infections: Insights from Coagulase Typing, Toxin Genes, and Antibiotic Resistance Patterns

Table 1

The PCR conditions and oligonucleotide primers used in this study.

Gene	Primer	Sequence	PCR condition (35 cycles)				Reference
			Denaturation (seconds)	Annealing (seconds)	Extension (seconds)	Product size (bp)
fem	F	CTT​ACT​TAC​TGC​TGT​ACC​TG	94°C, 50	55°C, 60	72°C, 50	648	[1]
	R	ATCTCGCTTGTTGTGTGC
nuc	F	GCG​ATT​GAT​GGT​GAT​ACG​GTT	95°C, 45	56°C, 50	72°C, 55	270	[2]
	R	AGC​CAA​GCC​TTG​ACG​AAC​TAA​AGC
mecA	F	AGA​AGA​TGG​TAT​GTG​GAA​GTT​AG	94°C, 45	55°C, 50	72°C, 50	583	[1]
	R	ATG​TAT​GTG​CGA​TTG​TAT​TGC
pvl	F	TTC​ACT​ATT​TGT​AAA​AGT​GTC​AGA​CCC​ACT	95°C, 60	57°C, 60	72°C, 60	180	[3]
	R	TAC​TAA​TGA​ATT​TTT​TTA​TCG​TAA​GCC​CTT
tst	F	TTA​TCG​TAA​GCC​CTT​TTG​TTG	95°C, 60	56°C, 60	72°C, 60	398	[3]
	R	TAA​AGG​TAG​TTC​TAT​TGG​AGT​AGG
eta	F	GCA​GGT​GTT​GAT​TTA​GCA​TT	94°C, 45	57°C, 45	72°C, 50	93	[2]
	R	AGA​TGT​CCC​TAT​TTT​TGC​TG
etb	F	ACA​AGC​AAA​AGA​ATA​CAG​CG	94°C, 45	56°C, 45	72°C, 55	226	[2]
	R	GTT​TTT​GGC​TGC​TTC​TCT​TG

F: forward; R: reverse. For all samples, initial denaturation of 95°C for 5 minutes and final extension of 72°C for 5 minutes were performed.