Ethanol Extract of Ampelopsis brevipedunculata Rhizomes Suppresses IgE-Mediated Mast Cell Activation and Anaphylaxis

Figure 4

Effects of ABE on inflammatory cytokine expression and NF-κB activation in mast cells. Anti-DNP IgE-sensitized RBL-2H3 were pretreated with/without drugs (ABE or Dex) for 1 h and stimulated with DNP-HSA. (a) After 1 h, the expression of TNF-α and IL-4 was determined using qPCR. The graph data represent the relative target gene expression. (b) After 6 h, inflammatory cytokine secretion was measured using ELISA. The graph data represent the mean ± SEM. (c) Western blot images show bands for IκBα and NF-κB obtained from cytoplasmic and nuclear protein extraction, respectively. β-Actin and Lamin B1 were used as loading controls. compared with the DNP-HSA-stimulated group. Dex: dexamethasone.

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