Review Article
Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus
Table 1
Yields of SHI from different expression systems.
| Protein | Expression host | Affinity tag | Purification steps | Protein yield (mg)1 | Reference |
| Native SHI | P. furiosus | — | 4 | 2.5 | [6] | Recombinant SHI | E. coli | — | 3 | 0.16 | [10] | Strep-tag II SHI | P. furiosus | Strep-tag II | 1 | 17 | [11] | 9x-His Dimeric SHI | P. furiosus | 9x-His tag | 2 | 16 | [13] | 9x-His SHI | P. furiosus | 9x-His tag | 1 | 135 | [12] |
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Protein yield from 100 g of cells (wet weight).
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