Osteometric Effects of Surgical Caponisation on Some Long Bones in Cockerel Chickens

Mahmud, Muhammad Abdullahi; Shaba, Peter; Gana, James; Yisa, Helen Yarubi; Ndagimba, Ruth; Abdulsalam, Wosilat; Ndagi, Silas; Abubakar, Habiba Lami

doi:https://doi.org/10.1155/2014/645071

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Research Article | Open Access

Volume 2014 | Article ID 645071 | https://doi.org/10.1155/2014/645071

Osteometric Effects of Surgical Caponisation on Some Long Bones in Cockerel Chickens

Muhammad Abdullahi Mahmud,¹Peter Shaba,¹James Gana,²Helen Yarubi Yisa,¹Ruth Ndagimba,¹Wosilat Abdulsalam,¹Silas Ndagi,¹and Habiba Lami Abubakar³

Academic Editor: Rick Hochberg

Received08 Apr 2014

Revised23 Jun 2014

Accepted30 Jun 2014

Published07 Jul 2014

Abstract

The study was conducted to assess the osteometric effects of surgical caponisation on long bones of cockerel chickens. Sixty- (60-) day-old chicks were distributed into two experimental groups with thirty (30) cockerels per group. The birds were caponised at eight (8) weeks of age. The mean of final body weights of caponized groups was significantly higher () than the uncaponised group. The weights of all long bones measured as well as lengths between the two groups were not statistically different () from one another except the weight of femur of the caponized group and the lengths of tibia and tarsometatarsus () that differed significantly from one another (). All the proximal, midshaft, and distal diameters of all the long bones measured between the two groups were not statistically different () from one another except the midshaft diameter of ulna that was significantly higher () in caponized group. It was concluded that caponisation of cockerel chickens at eight (8) weeks of age has no significant osteometric effects () on almost all the long bones studied when they were normalised to the final body weights.

1. Introduction

Surgical caponisation is defined as a process whereby the testes are artificially removed via a surgical operation on 6- to 12-week-old chickens [1]. It results in androgen deficiency and thus secondary male sexual characteristics such as the comb, the wattle, fighting behavior, and vocalisation degeneration [2]. It is well known that the abdominal fat pad is significantly increased in capons, regardless of the breed and the age of caponisation or slaughter [3, 4].

There are conflicting reports on the effects of caponisation on the growth of chickens. Several studies have demonstrated that caponisation enhanced chicken growth [5–7] and has no effects on growth [2, 8, 9] or even negative results [10]. Androgen has long been recognized as playing an important role in bone development, physiology, and metabolism [11, 12]; increased growth rate is often achieved after caponisation and likely as a result of androgen deficiency [2]. In humans, Manolagas et al. [13] reported that depressed androgen through exposure to chemicals, testectomy operation, or age has adverse effects on bone growth and development.

In poultry, however, very few experiments conducted on the effects of caponisation on the bones of chickens have shown consistent results. For example, Hutt [14] found increased bone length on the caponised white leghorn while Chen et al. [1] and Landauer [15] found no significant effects of caponisation on bone length of male chickens. Still, several studies have found negative responses to caponisation [16–22]. Therefore, the aim of this study was to generate baseline data on the osteometric effects of surgical caponisation on some long bones in cockerel chickens and to add to the few available data on its effects on the bones of chicken in general.

2. Materials and Methods

2.1. Birds Management and Experimental Design

The present study was conducted in the poultry unit, Niger State College of Agriculture farm Mokwa. Sixty- (60-) day-old chicks were purchased from Zarm farm Ilorin, Nigeria, and were randomly distributed into two experimental groups (caponized and uncaponized groups) with thirty (30) cockerels per group. The birds were given water and commercial starter diet ad libitum from week one (1) to week six (6) and then commercial grower diet (vital feed) from week seven (7) till the end of the experiment at week sixteen (16).

2.2. Caponisation

All the cockerels in the caponized group were caponized at eight (8) weeks of age. The birds were deprived of feed and water overnight before the procedure. Anaesthesia was performed using a combination of ketamine dosed at 20 mg/kg live body weight and diazepam dosed at 1 mg/kg live body weight. After the removal of the feathers and disinfection of the skin with methylene spirit, 1.5 cm incision was made between the two (2) last ribs. A ribs retractor was inserted and the membranes were cleared with groove director. The testicles were then removed. The site was then re-disinfected and left unstitched.

2.3. Slaughter Procedure

The birds were fastened 24 hours and slaughtered using Halal method [23]. They were allowed to bleed for two (2) minutes before being defeathered. The limbs were then harvested. The soft tissue attached to all the long bones harvested such as muscles, fascia, tendons, and ligaments which were removed using the scalpel.

2.4. Measurement of the Bones

All weights were measured in grams using a sensitive electronic balance (Mettler balance P 1210, Mettler instrument AG. Switzerland; sensitivity: 0.001 g). The lengths of all the bones were measured in centimetres using a thread. The readings were taken by stretching the measured length of the thread against a ruler. Diameters of the bones extremities and midshaft were measured in (mm) using vernier caliper.

2.5. Statistical Analysis

All recorded weights, lengths, and diameters were expressed as mean ± SEM (standard error of mean) and subjected to statistical analysis using statistical package for social sciences (SPSS) version 17.0. Independent sample -test at 95% confidence interval was used to determine the level of significant difference in mean values between the two groups. Values of () were considered significant.

3. Results

Table 1 presented the mean final body weight and mean weights of long bones in the two groups (caponized and uncaponized). The results indicated that the mean final body weights of the caponized group were significantly higher () than those of the uncaponized group. The mean weights of ulna, tibia, and humerus between the two groups were not significantly different () from one another. However, the mean weight of femur of caponized group was significantly higher () than that of the uncaponized group.

Table 2 presented the mean lengths of humerus, femur, ulna, tibia and tarsometatarsus in the two groups. The mean lengths of all the measured bones were not statistically higher () than those of the uncaponized group. However, the mean lengths of tibia and tarsometatarsus of the caponized group were significantly higher () than those of the uncaponized group.

Table 3 presented the mean diameters of the proximal extremities of the long bone in the two groups. The differences in diameter of the proximal extremities of all the long bones studied in the two groups were not significantly different () from one another.

Table 4 presented the mean diameter of the midshafts of the long bones in the two groups. With the exception of the midshaft diameter of ulna that was significantly higher () in the caponized group, all other diameters of the midshaft of humerus, femur, tibia, and tarsometatarsus of the two groups were not significantly different () from one another.

Table 5 presented the mean diameters of the distal extremities of the long bone in the two groups. The mean diameters of the distal extremities of all the studied bones in the two groups were not significantly different () from one another.

4. Discussion

All the data recorded on the long bones were normalised to the body weight of the cockerel chickens. The statistical difference observed in the weights of femur might be as a result of age of caponisation in this study (8 weeks) since the target cells of the effects of sex steroids on bone growth are different from the target cells of their effects on the maintenance of the mature skeleton [13]. The present result is not consistent with an earlier study in castrated young male rats, whose skeletal growth was delayed even when they were castrated after puberty [24]. However, this observed difference might be due to difference in species and nutritional level [5, 25–27].

The statistical significance observed in the lengths of tibia and tarsometatarsal bone study might be due to the age of caponisation since in the mature skeleton the change in bone size with time is trivial [13]. The present study is in line with the findings of Hsieh [6] who found a significant difference in the tibia length of Taiwan cockerel chicken and Chen et al. [1] who reported that the tarsometatarsal perimeter in the capons was higher than uncaponised chicken. This finding is not in agreement with other studies that showed no influence [8, 17] or decreased tibia length [28].

The nonsignificant difference found in the lengths of other bones was in line with the finding of an earlier study by Landauer [15] who did not find a significant effect on bone length in caponized males chickens fed up to 10 months of age, while it was not consistent with the finding of Hutt [14] who reported that caponized white leghorn chickens increased bone length.

Generally, though it is not documented to our knowledge, the osteometric effects of surgical caponisation on the long bone diameters in birds but androgen has been reported to influence mammalian bone development [29]. Therefore, its depression through chemical, testectomy operation or age has adverse effect on bone growth and development in human beings [13] as well as in bone growth of broiler chickens [19–22].

5. Conclusion

Based on this study, surgical caponisation of cockerel chickens at eight (8) weeks of age has no significant osteometric effects () on almost all the parameters of the long bones studied when they were normalised to the final body weights.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

References

K. L. Chen, T. Y. Hsieh, and P. W. Chiou, “Caponization effects on growth performance and lipid metabolism in Taiwan country chicken cockerels,” Asian-Australasian Journal of Animal Sciences, vol. 19, no. 3, pp. 438–443, 2006.
View at: Google Scholar
K. L. Chen, W. T. Chi, and P. W. S. Chiou, “Caponization and testosterone implantation effects on blood lipid and lipoprotein profile in male chickens,” Poultry Science, vol. 84, no. 4, pp. 547–552, 2005.
View at: Publisher Site | Google Scholar
J. A. Cason, D. L. Fletcher, and W. H. Burke, “Research note: effects of caponization on broiler growth,” Poultry Science, vol. 67, no. 6, pp. 979–981, 1988.
View at: Publisher Site | Google Scholar
M. Tor, J. Estany, D. Villalba, E. Molina, and D. Cubiló, “Comparison of carcass composition by parts and tissues between cocks and capons,” Animal Research, vol. 51, no. 5, pp. 421–431, 2002.
View at: Publisher Site | Google Scholar
M. G. Mast, H. C. Jordan, and J. H. Macneil, “The effect of partial and complete caponization on growth rate, yield and selected physical and sensory attributes of cockerels,” Poultry Science, vol. 60, no. 8, pp. 1827–1833, 1981.
View at: Publisher Site | Google Scholar
T. Y. Hsieh, Effects of castration in Taiwan country chicken cockerels on growth performance and lipid metabolism [M.S. thesis], National Chung-Hsing University, Taichung, Taiwan, 2003.
M. A. Mahmud, P. Shaba, J. Gana, H. Y. Yisa, and R. Ndagimba, “Effects of surgical caponisation on growth, carcass and some haematological parameters in cockerel chickens,” Sokoto Journal of Veterinary Sciences, vol. 11, no. 2, pp. 57–62, 2013.
View at: Google Scholar
M. J. Fennell and C. G. Scanes, “Inhibition of growth in chickens by testosterone, 5 alpha-dihydrotestosterone, and 19-nortestosterone,” Poultry Science, vol. 71, no. 2, pp. 357–366, 1992.
View at: Publisher Site | Google Scholar
H. T. Wang, Effects of surgical caponization and administration of Estradiol to chicken embryos on daily activity, agonistic behaviour, growth performance, carcass traits and sensory evaluation of males Taiwan country chickens [M.S. thesis], Department of Animal Science, National Chung-Hsing University, Taichung, Taiwan, 2001.
T. W. Kuo, Effect of different caponized ways and different caponized weeks on meat characteristics of Taiwan country chicken [M.S. thesis], Department of Animal Science, National Chung-Hsing University, Taichung, Taiwan, 2002.
M. J. Oursler, M. Kassem, B. L. Riggs, and T. C. Spelsberg, “Regulation of bone cell function by gonadal steroid,” in Osteoporosis, R. Marcus, D. Feldman, and J. Kelsey, Eds., pp. 237–260, Academic Press, New York, NY, USA, 1996.
View at: Google Scholar
L. Pederson, M. Kremer, J. Judd et al., “Androgens regulate bone resorption activity of isolated osteoclasts in vitro,” Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 2, pp. 505–510, 1999.
View at: Publisher Site | Google Scholar
S. C. Manolagas, S. Kousteni, and R. L. Jilka, “Sex steroids and bone,” Recent Progress in Hormone Research, vol. 57, pp. 385–409, 2002.
View at: Publisher Site | Google Scholar
F. B. Hutt, “Sex dimorphism and variability in the appendicular skeleton of the leghorn fowl,” Poultry Science, vol. 8, no. 4, pp. 202–218, 1929.
View at: Google Scholar
W. Landauer, “Studies on the creeper fowl, X1 castration and length of the appendicular skeleton of in normal and creeper fowl,” The Anatomical Record, vol. 69, no. 2, pp. 247–253, 1937.
View at: Google Scholar
S. Leeson, J. D. Summers, and J. P. Walker, “The effect of light and diet on performance and carcass quality of capons,” Poultry Science, vol. 55, no. 1, pp. 438–440, 1976.
View at: Publisher Site | Google Scholar
Y. Ono, H. Iwamoto, H. Takahara, and M. Okamoto, “Studies on the growth skeletal muscle of capon. 1. Effects of castration on the weights of skeletal muscle, abdominal fat, intramuscular fat, skin, bone and viscera,” Science Bulletin of Faculty of Agriculture, Kyushu University, vol. 34, pp. 39–46, 1979.
View at: Google Scholar
A. L. Johnson and V. T. Rendano, “Effects of castration, with and without testosterone replacement, on leg bone integrity in the domestic fowl,” American Journal of Veterinary Research, vol. 45, no. 2, pp. 319–325, 1984.
View at: Google Scholar
N. C. Rath, W. E. Huff, J. M. Balog, and G. R. Bayyari, “Effect of gonadal steroids on bone and other physiological parameters of male broiler chickens,” Poultry Science, vol. 75, no. 4, pp. 556–562, 1996.
View at: Publisher Site | Google Scholar
N. C. Rath, G. R. Huff, W. E. Huff, and J. M. Balog, “Factors regulating bone maturity and strength in poultry,” Poultry Science, vol. 79, no. 7, pp. 1024–1032, 2000.
View at: Publisher Site | Google Scholar
C.-Y. Lin and J.-C. Hsu, “Comparison of some selected growth, physiological and bone characteristics of capon, slip and intact birds in Taiwan country chicken cockerels,” Asian-Australasian Journal of Animal Sciences, vol. 16, no. 1, pp. 50–56, 2003.
View at: Google Scholar
K. Chen, S. Tsay, D. Lo, F. Kuo, J. Wang, and P. W. Chiou, “Effects of caponization and testosterone on bone and blood parameters of SCWL male chickens,” Asian-Australasian Journal of Animal Sciences, vol. 20, no. 5, pp. 706–710, 2007.
View at: Google Scholar
G. W. Wilson, Wilson’s Practical Meat Inspection, Blackwell, Malden, Mass, USA, 4th edition, 2005.
H. Spencer, J. A. Friendland, and I. Lewin, Anabolic-Androgenic Steroids (Hand Book of Experimental Pharmacology), vol. 43, 1976.
J. C. Layfield, W. J. Owings, S. L. Balloun, and D. L. Miller, “Carcass composition and production criteria of surgical capons as affected by nutrition,” Poultry Science, vol. 51, no. 5, p. 1597, 1971.
View at: Google Scholar
J. F. Welter, “The effects of surgical caponization on production efficiency and carcass yield of roosters,” Poultry Science, vol. 55, no. 4, pp. 1372–1375, 1976.
View at: Google Scholar
K. L. Chen, C. P. Wu, and R. G. R. Chou, “Effect of caponization age on growth performance and post-mortem change in muscles of Taiwan country chicken,” Journal of Agricultural Association of China, vol. 1, no. 1, pp. 54–63, 2000.
View at: Google Scholar
S. M. Tsay, K. L. Chen, and P. W. S. Chiou, “Testosterone implantation on the bone characteristics in castrated male chickens,” in Proceeding of 11th AAAP Animal Science Congress , Kuala Lumpur, Malaysia. Asian-Australian Association of Animal Production Society, pp. 321–324, Kuala Lumper , Malaysia, 2004.
View at: Google Scholar
K. L. Chen, T. T. Chen, K. J. Lin, and P. W. S. Chiou, “The effects of caponization age on muscle characteristics in male chicken,” Asian-Australasian Journal of Animal Sciences, vol. 20, no. 11, pp. 1684–1688, 2007.
View at: Google Scholar

Copyright

Copyright © 2014 Muhammad Abdullahi Mahmud et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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