Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase from Pseudomonas stutzeri AS22

Table 1

Effects of different carbon sources on the production of α-amylase by P. stutzeri AS22.

Carbon sources	Final pH value	Biomass (OD600)	α-Amylase activity (U/mL)
Lactose	7.49	0.1	0.055
Glucose	5.69	0.8	0.1
Maltose	6.62	1.0	0.5
Potato starch	6.52	0. 8	0.8
Wheat starch	6.69	1.04	0.1
Maize starch	6.59	0.5	0.17

Cultivation was performed for 24 h at 37°C with shaking at 200 rpm in media that consisted of (g/L) carbon source 10, ammonium sulphate 1, K₂HPO₄ 1.4, KH₂PO₄ 0.7, MgSO₄ 0.1, and NaCl 0.5 and that were inoculated at initial OD of 0.016 and adjusted to pH 8.0.