Research Article
Highly Effective Renaturation of a Streptokinase from Streptococcus pyogenes DT7 as Inclusion Bodies Overexpressed in Escherichia coli
Table 1
Effect of surfactant, glycerol, and temperature on the renaturation of cell lysate E. coli/pESK.
| Parameter | SK activity (U/mL) | at 37°C for 1 h | at 37°C for 1 h + 10% glycerol | at 4°C for 6 h + 10% glycerol |
| 0.5% Triton X-100 | 643.4 ± 3.6 | 5574.0 ± 36.2 | 7591.3 ± 45.2 | 1% Tween 20 | 657.6 ± 5.7 | 4592.8 ± 27.1 | 6587.7 ± 27.1 |
1.5% Tween 80 | 691.6 ± 6.6 | 5226.8 ± 50.6 | 6578.7 ± 63.3 | No surfactant | 182.1 ± 1.8 | 184.3 ± 3.6 | 189.4 ± 3.6 |
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