The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases

Figure 4

AFM image of negatively supercoiled pBR322 in the different concentrations of DMSO. (a) Untreated pBR322. (b) pBR322 in 5% DMSO. (c) pBR322 in 10% DMSO. The black arrows indicate the single-stranded regions in plasmid.

(a)

(b)

(c)