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BioMed Research International
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2015
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Article
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Tab 1
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Research Article
MALDI-TOF MS and CD Spectral Analysis for Identification and Structure Prediction of a Purified, Novel, Organic Solvent Stable, Fibrinolytic Metalloprotease from
Bacillus cereus
B80
Table 1
Purification steps for metalloprotease.
ā
Activity (U)
Protein (mg)
Sp. activity U/mg
Recovery
Fold purification
Crude enzyme
31773.5
117.12
271.29
100
1
EtOH treated
31773.1
87.2
364.76
100
1.34
Sephadex
15682.7
10.4
1507.95
49.35
5.55
Q Sepharose
10668.9
3
3556.3
33.577
13.10