Effect of Chemotherapeutics and Tocopherols on MCF-7 Breast Adenocarcinoma and KGN Ovarian Carcinoma Cell Lines In Vitro

Figure 2

Effect of 4-Cyc on cell viability. (a) MCF-7 and (b) KGN cells were exposed to 4-Cyc 0, 0.5, 1, 2.5μM for 24h exposure (24H+), 24h exposure and 24h culture with media (24H+24H-), 24h exposure and 48h culture with media (24H+48H-), or 72h continuous exposure (72H+). Complete RPMI or DMEM/F12 without 4-Cyc (0μM) was used as a control. Cell viability was assessed by a crystal violet assay, in which cell number was obtained by comparison with a standard curve and % cell viability was calculated from medium control. Means ± SD of 3 independent experiments shown. Data analysed by one-way ANOVA with Tukey’s post hoc test.

(a) MCF-7 cells

(b) KGN cells