Effect of Chemotherapeutics and Tocopherols on MCF-7 Breast Adenocarcinoma and KGN Ovarian Carcinoma Cell Lines In Vitro

Figure 5

Cytotoxicity of combined chemotherapeutic regime. (a) MCF-7 and (b) KGN cells were exposed to a combination of chemotherapeutics (25μM Dox + 2.5μM 4-Cyc), or a combination of chemotherapeutics + 75μM αToc, or a combination of chemotherapeutics + 75μM γToc for 24h (24H+); 24h exposure and 24h culture with media (24H+24H-); 24h exposure and 48h culture with media (24H+48H-); or 72h continuous exposure (72H+). Cell viability was assessed by a crystal violet assay, in which cell number was obtained by comparison with a standard cue and % cell viability was calculated from vehicle control. Means ± SD of 3 independent experiments shown. Data analysed by one-way ANOVA with Tukey’s post hoc test. p ≤ 0.05; p ≤ 0.01, p ≤ 0.0001 compared to control same concentration of doxorubicin alone (25μM).

(a) MCF-7 cells

(b) KGN cells