Transcriptional Activation of p53 during Cold Induced Torpor in the 13-Lined Ground Squirrel Ictidomys tridecemlineatus

Figure 1

p53 protein expression and posttranslational modifications in the ground squirrel skeletal muscle over six stages of the torpor cycle. Stages of torpor are EC (euthermic in cold room), EN (entry into torpor), ET (early torpor), LT (late torpor), EA (early arousal), and IA (interbout arousal); see Section 2 for more details. Relative protein levels and Western blots are shown for total p53, p53 phosphorylated at serine 15, 46, and 392, p53 acetylated at lysine 382, and the housekeeping protein GAPDH. Data show means ± SEM, -5 independent trials on tissues from different animals. denotes significant statistical difference from EC values, .