Research Article
Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice
Table 5
Effect of NAC and GSH pretreatment on ROS generation and apoptosis in A375 cells.
| ||||||||||||||||||||||||||||||||||||||||||||
A375 cells were divided into 2 groups: one was pretreated with NAC or GSH for 1 hour and then treated with p-PD for 16 hours and the other was treated with only p-PD. ROS values and apoptotic cell numbers were quantified at the end of p-PD exposure as mentioned in the Materials and Methods. Percentage changes in ROS and apoptosis were calculated as follows: % change = (only p-PD value − respective pretreatment value)/respective pretreatment value × 100. Values are representative of three independent experiments. |