Preclinical Molecular PET-CT Imaging Targeting CDCP1 in Colorectal Cancer

Figure 1

Cell-based and mouse xenograft assays identify HCT116 cells for assessment of CDCP1-directed radioligand molecular imaging for CRC. (a) Flow cytometry evaluation of cell surface CDCP1 expression on HCT116, SW480, and HT29 CRC cell lines, positive control prostate cancer PC3 cells, and negative control ovarian cancer OVMZ6 cells. Cells were stained with anti-CDCP1 antibody 10D7 then Alexa Fluor 488 tagged goat-anti-mouse secondary antibody. Cells stained only with the secondary antibody provided background signal. Signals were normalised to mode. (b) Western blot analysis for CDCP1 of lysates from HCT116 cells using anti-carboxyl terminal antibody 4115. Arrowheads indicate full-length 135 kDa and cleaved 70 kDa CDCP1. An incompletely N-glycosylated form of CDCP1 is indicated by the arrow.

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