Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1

Figure 4

The changes in the protein expression levels of HIF-1α, Pit-1, Runx2, and SM22α. Western blot analysis was employed to examine the protein expression levels of HIF-1α, Pit-1, Runx2, and SM22α on day 7. The levels were normalized to those of tubulin. The data shown are indicative of mean ± SD. vs. CNT; ^# vs. HP. CNT, HASMCs cultured under normal conditions with 0.9 mM Pi; HP, HASMCs cultured with 2.5 mM Pi; HPSI, HIF-1α siRNA-transfected HASMCs cultured with 2.5 mM Pi; HPOE, HIF-1α overexpression plasmid-transfected HASMCs cultured with 2.5 mM Pi. HIF-1α, hypoxia-inducible factor 1 alpha; Pit-1, type III sodium-dependent phosphate cotransporter 1; Runx2, runt-related transcription factor 2; SM22α, smooth muscle 22 alpha; SD, standard deviation; CNT, control; HP, high Pi; HASMCs, human aortic smooth muscle cells; Pi, inorganic phosphorous; HPSI, siRNA and high Pi; HPOE, overexpression and high Pi.