A Degenerate Primer Design Method Based on Ant Colony Optimization

Table 3

An analysis of DNA sequences containing 100 bps, 200 bps, 300 bps, and 400 bps.

	100 bps	200 bps	300 bps	400 bps
Up primer (3′- 5′)	CG[TG]T[TA]	G[CT]A[GT]	G[GA]G[AG]AG	GG[GA]A[CA]C
	[GA]G[GA]T[CA]C	[TC]GG[CA]A[GT]	[CT][GC]A[GA]A	[AC]A[TC]ACG[TG]
	[GC]T[TG]C	[TG]CATT[CA]	[GT]C[CT]GT[TA]	[CT][GA][GA]C
	[GA]GT[GC]A	AG[TA]	[GTA]A[CA]	[CGT]A[CT]
Down primer (3′- 5′)	GG[GT][GT]AA	GT[GA][GT]TG	G[GA]G[AG]T[CTG]	G[AG][GA]CGA
	[GT][GT]CC[CT]	[AG]A[CG]TGA[GA]	[GA][AC]G[CA]T	[ACG]T[AC][AC][GA]
	ATG[TA]TG	[GT][GT]AG	[AC]C[TG]T	G[CT]AA
	[CG]T[CA]	[GA]G	[CA]A[GA]A	[CTA]T[AC]T