Ganoderma tsugae Induces S Phase Arrest and Apoptosis in Doxorubicin-Resistant Lung Adenocarcinoma H23/0.3 Cells via Modulation of the PI3K/Akt Signaling Pathway
Figure 2
Effect of GTE on cell viability in doxorubicin-resistant lung adenocarcinoma H23/0.3 cells. (a) H23 and H23/0.3 cells were treated with either vehicle control or GTE (0.2 mg/mL, 0.4 mg/mL, 0.6 mg/mL, or 0.8 mg/mL) for 72 h. Cell viability was determined using the MTT assay as described in the Section 2. (b) H23 and H23/0.3 cells were treated with various concentrations of doxorubicin (0.01–0.1 μg/mL in H23 cells; 0.5–3 μg/mL in H23/0.3 cells) for 72 h. Cell viability was determined using the MTT assay. (c) H23/0.3 cells were treated with various concentrations of doxorubicin (0 μg/mL, 0.25 μg/mL, 0.5 μg/mL, 1 μg/mL, 3 μg/mL, and 6 μg/mL) with or without GTE (0.1 mg/mL or 0.2 mg/mL) for 72 h. Cell viability was determined by the MTT assay. Results are expressed as the mean ± SD of three independent experiments.
