Ganoderma tsugae Induces S Phase Arrest and Apoptosis in Doxorubicin-Resistant Lung Adenocarcinoma H23/0.3 Cells via Modulation of the PI3K/Akt Signaling Pathway
Figure 4
Effect of GTE on cellular apoptosis in H23/0.3 cells. (a) H23/0.3 cells were treated with various concentrations of GTE (0 mg/mL, 0.3 mg/mL, and 0.9 mg/mL) for 24 h. Cellular apoptosis was measured by flow cytometry using the FITC-conjugated annexin V and PI double stains as described in Section 2. (b) H23/0.3 cells were treated with 0.9 mg/mL of GTE for 24 h. The exposure of phosphatidylserine (PS) was measured by confocal microscopy with annexin V-FITC (green color, stained for early asymmetry membrane) and PI (red color, stained for nuclear chromosome after cell membrane disruption) double stains. (c) H23/0.3 cells were treated with 0.9 mg/mL of GTE for 24 h. Fragmented condensed nuclei were measured using a DAPI staining assay.
