Ganoderma tsugae Induces S Phase Arrest and Apoptosis in Doxorubicin-Resistant Lung Adenocarcinoma H23/0.3 Cells via Modulation of the PI3K/Akt Signaling Pathway
Figure 6
Effect of GTE on PI3K/Akt pathways in H23/0.3 cells. (a) H23/0.3 cells were treated with various concentrations of GTE (0 mg/mL, 0.3 mg/mL, and 0.9 mg/mL) for 24 h and 48 h. The protein levels of p-Akt and Akt were measured by western blotting. (b) H23/0.3 cells were treated with 20 M LY294002 (a PI3K inhibitor) alone or in combination with GTE (0.3 mg/mL or 0.9 mg/mL) for 24 h and 48 h. The expression levels of proteins (cyclins A and E) were measured by western blotting. (c) A proposed model for the GTE-mediated antiproliferation of doxorubicin-resistant lung adenocarcinoma H23/0.3 cells. Results are expressed as the mean ± SD of three independent experiments.
