Explore the Molecular Mechanism of Apoptosis Induced by Tanshinone IIA on Activated Rat Hepatic Stellate Cells
Figure 4
(a) Cell viability was determined with or without RNA interference-mediated silencing of prohibitin under exposure to 7.5 μM Tan IIA. The quantified results were indicated by the bar chart. Results represent the mean±SD of three independent experiments (*). (b) Tan IIA enhanced prohibitin expression and C-raf translocation in HSC-T6 cells. The membrane and cytosolic fractions were prepared and subjected to western blot using a specific antibody. Na+/K+-ATPase α1 used as a specific membrane marker protein was not identified in cytosol fraction to confirm a complete separation of cell fractions. β-actin was used as an internal control.
