Xanthohumol, a Prenylated Flavonoid from Hops (Humulus lupulus), Prevents Platelet Activation in Human Platelets

Figure 1

Effects of xanthohumol (XN) on the inhibition of platelet aggregation and relative [Ca²⁺]_i mobilization in activated platelets. Washed platelets (3.6×10⁸ cells/mL) were preincubated with 1.5~10 μM XN or a solvent control (0.5% DMSO), followed by the addition of 1 μg/mL collagen (◯), 1 μM U46619 (▿), 60 μM arachidonic acid (AA; □), or 0.05 IU/mL thrombin (◊) to trigger ((a)-(b)) platelet aggregation and an ATP-release reaction ((a), top-left corner) or (c) relative [Ca²⁺]_i mobilization. Profiles (a) are representative examples of six similar experiments. Data ((b) and (c)) are presented as the means ± S.E.M. ((b), 𝑛=6; (c), 𝑛=3); **𝑃<0.01, compared to the resting group; ^##𝑃<0.01, compared to the control (DMSO) group.

(a)

(b)

(c)