Baicalin’s Therapeutic Time Window of Neuroprotection during Transient Focal Cerebral Ischemia and Its Antioxidative Effects In Vitro and In Vivo

Figure 5

Protection effects of baicalin against H₂O₂-induced cell toxicity in primary rat cortical neuronal cultures. (a) The cell viability of primary rat cortical neuronal cultures injured by different concentrations of H₂O₂ after incubation for 16 h. An H₂O₂ concentration of 300 μM was chosen for the following experiments. (b) The effects of baicalin at concentrations of 10, 20, 40, 80, and 200 μM on the viability of H₂O₂ (300 μM) injured neurons. (c) Baicalin reduced the levels of LDH in the cell supernatant. (d) Baicalin at 80 and 200 μM increased the SOD activity of neuronal cultures. ^ΔΔ𝑃<0.01, H₂O₂-treated groups versus control; **𝑃<0.01,*𝑃<0.05, baicalin-treated groups versus H₂O₂-only (𝑛=8 for each group).

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