Neuromuscular Damage and Repair after Dry Needling in Mice
Figure 1
Experimental design and sampling. (a)(i) Levator auris longus (LAL) muscle in which repeated punctures are being made with a 0.16 mm thick needle. (a)(ii) Two LAL muscles obtained from the same mouse. (b) Techniques performed and experimental design. After DN, the animals were sacrificed on days one (three hours), two, three, five, and seven. After sacrifice, the muscles were removed, and histological techniques were performed as shown in the figure. In order to minimize the number of animals sacrificed and expenses, the days of extraction and the techniques used were chosen based on a preliminary pilot study.
