Neuromuscular Damage and Repair after Dry Needling in Mice
Figure 5
Muscle regeneration. (a) Myotubes. On the fifth day after puncture, myotubes involved in myofibrillar synthesis can be viewed. Note that there are many areas with newly synthesized contractile apparatus. Some myoblasts have not merged to myotube cells and will become satellite cells in the next days. (b) Young muscular fibers. Seven days after puncture, the DN, signs of degeneration and necrosis are less evident than on day five, and some residual inflammatory cells can be seen. As on the fifth day, some myoblasts attached to the young muscle fibers start regressing to satellite cells. Note that the cytoplasm of the muscle fibers is filled by the contractile apparatus. Some myonuclei definitely remain centralized. Methylene blue stain. Sample included in Spurr. Semithin of 1 µm. Initial magnification: 1000x.
