Ampelopsis Radix Protects Dopaminergic Neurons against 1-Methyl-4-phenylpyridinium/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-Induced Toxicity in Parkinson’s Disease Models In Vitro and In Vivo
Figure 5
Protective effects of AJW on dopaminergic neuronal loss by MPTP in mice. Saline or AJW at 10 or 100 mg/kg/day was administered orally for 7 days, and MPTP 20 mg/kg was injected intraperitoneally four times at 2 h intervals on the last day of AJW treatment. After the behavioral test, dopaminergic neurons were determined using TH-immunohistochemistry. The numbers of TH-positive neurons in the SNc were counted (a) and the optical density of TH positive fibers in the striatum was measured (b). Representative images are shown of the SNc ((c)–(f)) and the striatum ((g)–(j)) of each group. Control group ((c) and (g)), MPTP-only treated group ((d) and (h)), MPTP + AJW 10 mg/kg/day treated group ((e) and (i)), and MPTP + AJW 100 mg/kg/day treated group ((f) and (j)). Scale bar = 100 μm ((c)–(f)) and 400 μm ((g)–(j)). Values are indicated as the mean ± SEM. *** compared with the control group, ### compared with the MPTP-only treated group.
