Microarray Analysis of mRNA and MicroRNA Expression Profile Reveals the Role of β-Sitosterol-D-glucoside in the Proliferation of Neural Stem Cell

Figure 3

Comparison of NSC Proliferation Promoted by BSSG, BFGF, and EGF. Comparison of NSC proliferation promoted by BSSG, bFGF and EGF was determined by CCK-8 assay and the data are plotted as percentages of control cell proliferation. Data are presented as Mean ± SD (𝑛=6); **𝑃<0.01, ***𝑃<0.001 compared with the control. NSCs proliferation was induced by adding bFGF (20 ng/mL, 𝑃<0.001), EGF (20 ng/mL; 𝑃<0.001), and BSSG at different concentrations (10 and 20 μM, 𝑃<0.01; 40 μM, 𝑃<0.001). The effect of BSSG on NSC proliferation at 40 μM was similar to those of bFGF and EGF.