Anthricin Isolated from Anthriscus sylvestris (L.) Hoffm. Inhibits the Growth of Breast Cancer Cells by Inhibiting Akt/mTOR Signaling, and Its Apoptotic Effects Are Enhanced by Autophagy Inhibition
Figure 1
Anthricin isolated from Anthriscus sylvestris (L.) Hoffm. inhibits cell growth and proliferation. (a) Analysis of cell viability in anthricin-treated MDA-MB-231 and MCF7 cells. Cells were treated with varying concentrations of anthricin for 12 or 24 h. Cell viability was evaluated using the cell counting kit-8 (CCK-8). Data are expressed as a percentage of the control and shown as mean ± SD () values. compared with vehicle-treated cells. compared with the group treated with anthricin for 12 h. (b) Reduced cell proliferation rate of breast cancer cells. The error bars represent the mean ± SD . (c) Induction of apoptosis by anthricin in breast cancer cells. The extent of apoptosis in MDA-MB-231 and MCF-7 cells treated with varying concentrations of anthricin for 24 h was analyzed by immunoblotting. (d) Cell cycle arrest by anthricin in breast cancer cells. The expression of cell-cycle-related proteins was analyzed by immunoblotting.
