Corni Fructus Containing Formulation Attenuates Weight Gain in Mice with Diet-Induced Obesity and Regulates Adipogenesis through AMPK
Figure 2
Effect of CDAP on the expression of transcription factors and adipocyte-specific genes in differentiation of 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of CDAP (0, 10, 50, and 100 μg/mL) for 6 days. The mRNA of PPAR-γ (a), C/EBP-α (b), and lipin-1 (c) was analyzed by real-time RT-PCR. Results were expressed relative to untreated cells after normalization to GAPDH mRNA. EGCG was used as a positive control. Values are mean ± S.D. of data from three separate experiments; each experiment was performed in triplicate. # versus undifferentiated control cells; * versus differentiated control cells.
