Corni Fructus Containing Formulation Attenuates Weight Gain in Mice with Diet-Induced Obesity and Regulates Adipogenesis through AMPK
Figure 4
Effect of CDAP and its constituents on phosphorylation of AMPK during 3T3-L1 differentiation. Postconfluent 3T3-L1 cells were differentiated in the presence or absence of Corni Fructus, Dioscoreae Rhizoma, Aurantii Fructus Immaturus, and Platycodonis Radix for 6 days. (a) Lipid accumulation was measured by an Oil Red O staining assay. (b) The cytotoxicity of each constituent, naringin and platycodin D, which are more effective herb’s constituents on lipid accumulation, was examined using an MTS assay. (c) Effect of CDAP on AMPK activation was compared with naringin and platycodin D, a major constituent of Aurantii Fructus Immaturus and Platycodonis Radix, respectively. Protein levels of phosphorylated AMPK (pAMPK) and total AMPK were determined by western blot analysis. The protein expression differences are normalized to AMPK-α. Values are mean ± S.D. of data from three separate experiments; each experiment was performed in triplicate. # versus undifferentiated control cells; * versus differentiated control cells.
