Si Shen Wan Inhibits mRNA Expression of Apoptosis-Related Molecules in p38 MAPK Signal Pathway in Mice with Colitis

Table 2

Primer sequences for RT-PCR (mouse).

Gene	Primer sequences	Annealing temperature (°C)	Products (bp)
GAPDH	F: 5′GGAAAGCTGTGGCGTGAT3′	59	308
	R: 5′AAGGTGGAAGAATGGGAGTT3′
caspase-3	F: 5′GCTGGACTGCGGTATTGAGA3′	59	142
	R: 5′CCATGACCCGTCCCTTGA3′
p38MAPK	F: 5′GACGAATGGAAGAGCCTGAC3′	59	260
	R: 5′AGATACATGGACAAACGGACA3′
TNF-𝛼	F: 5′CTCAGCCTCTTCTCATTCCT3′	59	101
	R: 5′ATTTGGGAACTTCTCCTCCT3′
Bcl-2	F: 5′TGGGATGCCTTTGTGGAAC3′	59	167
	R: 5′CATATTTGTTTGGGGCAGGTC3′
Bax	F: 5′TGCTACAGGGTTTCATCCAG3′	59	175
	R: 5′ATCCACATCAGCAATCATCC3′
c-fos	F: 5′TGCGTTGCAGACCGAGA3′	59	293
	R: 5′GAAACAAGAAGTCATCAAAGGG3′
p53	F: 5′TGCTGAGTATCTGGACGACA3′	59	166
	R: 5′CAGCGTGATGATGGTAAGG3′
c-jun	F: 5′GGCTGTTCATCTGTTTGTCTTCA3′	59	300
	R: 5′TTCTTTACGGTCTCGGTGGC3′
c-myc	F: 5′GCTCAAAGCCTAACCTCACAA3′	59	117
	R: 5′AAAGAAAGAAGATGGGAAGCA3′