Ginsenoside Rb1 Reduces Isoproterenol-Induced Cardiomyocytes Apoptosis In Vitro and In Vivo

Figure 3

Apoptotic rates in H9c2 cells by flow cytometry assays. H9c2 cells were incubated with Annexin V-FITC and PI and analyzed in flow cytometry. (a) Control. (b)–(g) represent each treatment group. 𝑋-axis indicated the numbers of Annexin V-FITC stained cells as FL-1. The 𝑌-axis indicated the numbers of PI strained cells as FL-3. The percentages indicated on the graph are the percent of double positive PI and annexin V-stained cells. (h) Statistical graph of annexin V-FITC/PI staining. The tripleexperiment of results was expressed as mean ± SD, ^b𝑃<0.01 versus control; ^d𝑃<0.01, ^c𝑃<0.05 versus ISO; ^e𝑃<0.01 versus Rb1+ISO; ^h𝑃<0.01 versus ISO+Rb1+C-1.

(a) Control

(b) ISO 

(c) ISO+Rb1

(d) ISO+H89

(e) ISO+Rb1+H89

(f) ISO+C-1

(g) ISO+Rb1+C-1

(h)