Inhibition of Neuroinflammation in LPS-Activated Microglia by Cryptolepine
Figure 9
Cryptolepine (CAS) inhibited LPS-induced nuclear translocation of NF-κB (a), but not IκB phosphorylation (b) in LPS-activated microglia. Cells were stimulated with LPS (100 ng/mL) in the presence or absence of CAS (2.5 and 5 μM) for 15 min. Nuclear extracts were analysed for levels of NF-κBp60, while lysates were analysed for phospho-IκB using an ELISA kit. All values are expressed as mean ± SEM for 3 independent experiments. Data were analysed using one-way ANOVA for multiple comparison with post hoc Student Newman-Keuls test. * in comparison with LPS control.
