[Retracted] Decursin Isolated from Angelica gigas Nakai Rescues PC12 Cells from Amyloid -Protein-Induced Neurotoxicity through Nrf2-Mediated Upregulation of Heme Oxygenase-1: Potential Roles of MAPK
Figure 3
Effect of D on Aβ -induced oxidative neurotoxicity and intracellular ROS generation in PC12 cells. Effect of D on Aβ -induced oxidative neurotoxicity in PC12 cells. (a) PC12 cells were pretreated with various concentrations of D for 3 h and then incubated with and without 25 μM Aβ for 24 h. Cell viability was estimated by the WST-8 assay. Effect of D on Aβ -induced intracellular ROS generation in PC12 cells. (b) PC12 cells were pretreated with various concentrations of D for 3 h and then incubated with and without 25 μM Aβ for 24 h. After that the cells were washed with PBS and then treated with 10 μM H2DCF-DA for 30 min. (c) PC12 cells were incubated with D (10μM) for the indicated time. They were then treated with 25 μM Aβ for 24 h and with 10 μM H2DCF-DA for 30 min. Intracellular ROS production was measured at the excitation wavelength of 485 nm and the emission wavelength of 530 nm using the fluorescence microplate reader. Each bar represents mean ± SD from three experiments with triplicates. * compared with control. ** compared with control. *** compared with control. # compared with the group treated by Aβ alone. ### compared with the group treated by Aβ alone.
