Rubi Fructus (Rubus coreanus) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells
Figure 1
Effect of RF on cell viability and lipid accumulation in 3T3-L1 cells. (a) 3T3-L1 cells were treated with RF at various concentrations (10–500 μg/mL) for 48 h. Cell viability was determined by the MTS assay. Postconfluent 3T3-L1 cells were differentiated in the absence or in the presence of RF (0, 10, 50, and 100 μg/mL) for 6 days. (b) Triglyceride content was quantified by measuring absorbance. EGCG was used as positive control. Assays were performed in duplicate for each concentration, and experiments were repeated at least three times. Data are expressed as means ± S.D. where was considered a statistically significant difference from the differentiated control. (c) Lipid droplets were measured by Oil Red O staining.
