Rubi Fructus (Rubus coreanus) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells
Figure 3
Effect of RF on the expression of PPARγ and C/EBPα in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μg/mL) for 6 days. (a) PPARγ and (b) C/EBPα mRNA expressions were evaluated by quantitative real-time RT-PCR. (c) PPARγ and (d) C/EBPα protein expressions were analyzed by Western blot analysis. Data are expressed as the means ± S.D. where was considered a statistically significant difference from the differentiated control.
