Rubi Fructus (Rubus coreanus) Inhibits Differentiation to Adipocytes in 3T3-L1 Cells
Figure 4
Effect of RF on the expression of aP2, resistin, and adiponectin in 3T3-L1 cells. Postconfluent 3T3-L1 cells were differentiated in the absence or presence of RF (0, 10, 50, and 100 μg/mL) for 6 days. (a) aP2, (b) resistin, and (c) adiponectin mRNA expression were evaluated by the quantitative real-time PCR. (d) aP2, resistin, and adiponectin protein levels were analyzed by Western blot analysis. GAPDH was used as internal controls. Data are expressed as means ± S.D. where was considered a statistically significant difference from the differentiated control.
