Cartilage Protective and Chondrogenic Capacity of WIN-34B, a New Herbal Agent, in the Collagenase-Induced Osteoarthritis Rabbit Model and in Progenitor Cells from Subchondral Bone
Figure 4
Effects of WIN-34B on chondrogenic differentiation of progenitor cells from rabbit subchondral bone. (a) Surface marker profiling of progenitor cells using flow cytometry. Cells were positive for CD73, SH-2, CD90, CD166, and STOR-1, and negative for CD45, CD34, and CD11b. (b) Chondrogenic induction of progenitor cells from subchondral bone cultured by WIN-34B treatment in chondrogenic medium. Progenitor cells from passage 2 of subchondral bone were seeded in 6-well plates at a concentration of 5 × 105 cells/cm2. After 24 h, cells were treated or nontreated with WIN-34B (10 μg/mL) and cultured for 7 days and 14 days. Each of the three samples was measured for profiling markers.
