Cartilage Protective and Chondrogenic Capacity of WIN-34B, a New Herbal Agent, in the Collagenase-Induced Osteoarthritis Rabbit Model and in Progenitor Cells from Subchondral Bone
Figure 5
Effects of WIN-34B on chondrogenic differentiation of IL-1β-stimulated progenitor cells from rabbit subchondral bone. (a) Histological analysis of WIN-34B by alcian blue staining of chondrogenic differentiation in IL-1β-treated progenitor cells. Control, IL-1β, WIN-34B 1 μg/mL, WIN-34B 10 μg/mL, and WIN-34B 20 μg/mL after 7 days of culture in chondrogenic differentiation media. Magnified view (×100). (b) Dose response of WIN-34B on the mRNA expression of chondrogenic markers. Chondrogenic differentiation of subchondral progenitor cells that were incubated for seven days with 1, 10, and 20 μg/mL of WIN-34B in the presence of IL-1β. qRT-PCR was then performed for type II α1 collagen, cartilage link protein, and aggrecan. (c) Inhibitory effects of WIN-34B on GAG and type II collagen degradation on chondrogenic differentiation of IL-1β-stimulated subchondral progenitor cells. GAG and type II collagen degradation are shown as a cumulative release into the culture medium. Values are the mean ± SEM. ### compared to the control group. *, *** compared to the IL-1β group. Data were obtained from at least three independent experiments.
