Cartilage Protective and Chondrogenic Capacity of WIN-34B, a New Herbal Agent, in the Collagenase-Induced Osteoarthritis Rabbit Model and in Progenitor Cells from Subchondral Bone

Table 1

Primers of targeted genes.

mRNA	Primers	Annealing Tm (cycle)
Type II α1 collagen	Fw: 5′-AAC ACT GCC AAC GTC CAG AT-3′	58°C (32)
	Rv: 5′-CTG CAG CAC GGT ATA GGT GA-3′
Type I α1 collagen	Fw: 5′-TGA CCT CAA GAT GTG CCA CT-3′	58°C (32)
	Rv: 5′-GGG AGT TTC CAT GAA GCC-3′
Cartilage link protein	Fw: 5′-GCG TCC GCT ACC CCA TCT CTA-3′	55°C (32)
	Rv: 5′-CTC TAA GGG CAC ATT CAC TT-3′
Aggrecan	Fw: 5′-GAG GTC GTG GTG AAA GGT GT-3′	58°C (32)
	Rv: 5′-GTG TGG ATG GGG TAC CTG AC-3′
GAPDH	Fw: 5′-GCT CTC CAG AAC ATC ACT CCT GCC-3′	58°C (30)
	Rv: 5′-CGT TGT CAT ACC AGG AAA TGA GCT T-3′

Fw: forward; Rv: reverse; GAPDH: glyceraldehyde-3-phosphate dehydrogenase Tm: temperature.