Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug
Figure 1
Cytotoxic effect of EEDS on A549 human lung cancer cells. (a) A549 was exposed to increasing concentrations (0–100 μg/mL) of EEDS for various time periods (24–96 h). Cell viability was determined based on mitochondrial enzyme activity as described in the Materials and Methods section. The relative cell growth at each drug dose was calculated by comparison with the vehicle control (0 μg/mL of EEDS, 0.5% DMSO) treatment. (b) A549 cells were exposed to increasing concentrations (0–20 μg/mL) of EEDS for 72 h and then incubated for an additional 10 days in the absence of EEDS (upper panel). Colonies stained with crystal violet were counted and expressed as relative survival (%) compared to the vehicle control (0.5% DMSO) treatment (lower panel). All data are presented as the mean ± S.D. of triplicate experiments. The differences between the vehicle control and treated groups were determined using Student’s t-test. ***. (c) Representative FITC-Annexin V/PI scatter plots for A549 cells following the EEDS treatment. A549 cells were exposed to EEDS (0 or 20 μg/mL) for 12 h or 24 h, and then subjected to FACS analysis of FITC-Annexin V and PI.
