Research Article

Trypanocidal Activity of Smallanthus sonchifolius: Identification of Active Sesquiterpene Lactones by Bioassay-Guided Fractionation

Figure 6

Effect of enhydrin, uvedalin, and polymatin B on T. cruzi amastigotes. J774 phagocytic cells were infected with transfected trypomastigotes expressing β-galactosidase (10 : 1 parasite : cell ratio). After removing free parasites, STLs were added at concentrations ranging from 0.1 to 50 μg/mL. Three days after infection, nonidet P40 and chlorophenol red-β-d-galactopyranoside (CPRG) were added and the galactosidase activity was determined at 590 nm. Values represent the mean ± SEM.
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