Xiao-Xu-Ming Decoction Protects against Blood-Brain Barrier Disruption and Neurological Injury Induced by Cerebral Ischemia and Reperfusion in Rats
Figure 1
Diagram showing the experimental design of the present study. The rats () were randomly divided into 5 groups. Different doses of XXMD were orally administered to rats in the XXMD-treated groups for 3 days prior to the operation until the end of the experiments. And the others were given the distilled water. The rats in the IR and XXMD-treated groups were subjected to reperfusion following 90 min of middle cerebral artery occlusion, while the rats in the sham group underwent the same surgical procedure without monofilament occlusion. Brain tissues were collected for subsequent investigations, such as TTC staining, evaluation of brain water content, BBB leakage and BBB ultrastructure changes, western blot analysis, and immunohistochemistry. In addition, behavioral tests were performed at the indicated time points.
