Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology and In Vitro and In Vivo Efficacy and Safety Characterisation

Table 5

Minimum bactericidal concentration (MBC) of propolis water extract (PWE) and propolis ethanolic extract (PEE) from Brazilian green propolis.

Microorganisms	MBC^a (𝜇g/mL)
	PEE	PWE
Staphylococcus aureus; ATCC 25923	346.25 ± 0.012*	136.67 ± 0.003
Staphylococcus aureus; ATCC 43300	173.13 ± 0.006*	68.33 ± 0.002
Staphylococcus epidermidis; ATCC 14990	346.25 ± 0.012*	136.67 ± 0.003
Streptococcus pneumoniae; ATCC 49619	43.28 ± 0.002	136.67 ± 0.003*
Escherichia coli; ATCC 25922	692.5 ± 0.024	1093.3 ± 0.023*
Haemophilus influenzae; ATCC 9006	43.28 ± 0.002*	17.03 ± 0.000

aMBC values (𝜇g/mL) expressed like total flavonoid content. *Significant difference between PEE and PWE (𝑃<0.001).
Values are presented as the mean ± SD (𝑛=3).