Hepatitis B Virus-Encoded X Protein Downregulates EGFR Expression via Inducing MicroRNA-7 in Hepatocellular Carcinoma Cells
Figure 3
HBx upregulated miR-7 expression to reduce EGFR protein level in HCC cells. (a) The miR-7 expression in HCC cells was examined by RT-qPCR. The miR-7 expression was normalized to small RNA U47 level. Statistical analysis was performed by Student’s -test. **; *** as compared to individual parental cells. (b) Hep3Bx cells were transiently transfected with si-control or si-HBx for 3 days. The miR-7 expression was analyzed by RT-qPCR. The miR-7 expression was normalized to small RNA U47 expression. Statistical analysis was performed by Student’s -test. ** as compared to control group. (c) Hep3B and Hep3Bx cells were transiently transfected with miR-7 mimic or miR-7 inhibitor, respectively. Four days later, the EGFR protein expression was analyzed by Western blot.
