Citrus bergamia Risso Elevates Intracellular Ca²⁺ in Human Vascular Endothelial Cells due to Release of Ca²⁺ from Primary Intracellular Stores

Figure 4

Involvement of mitochondrial Ca²⁺ uptake and SOC pathway on BEO-induced Ca²⁺ release. Inhibition of mitochondrial Ca²⁺ uptake and SR/ER Ca²⁺-ATPase on BEO-induced Ca²⁺ release with CCCP (20 μM) and BHQ (30 μM), respectively (a). Increase in [Ca²⁺]_i by subsequent application of BEO in the presence of BHQ and CCCP (black trace a) was higher than that in the presence of BHQ only (gray trace b). Effects of SOC pathway in BEO-induced intracellular Ca²⁺ release. SOC was induced by reintroduction of extracellular Ca²⁺ following SR/ER Ca²⁺-ATPase inhibition with BHQ (b). Intracellular Ca²⁺ levels were further enhanced by subsequent addition of BEO. Data are means ± SEMs. **𝑃<.01; 𝑛=15 cells/group. Applications of BEO or drugs are indicated by upper or bottom lines.

(a)

(b)