Ginseng Extracts Restore High-Glucose Induced Vascular Dysfunctions by Altering Triglyceride Metabolism and Downregulation of Atherosclerosis-Related Genes
Figure 1
HPLC fingerprint of PPT-type (Panel (a)) and PPD-type (Panel (b)) ginseng extract used in current study. Instruments. An HP 1100 system (Hewlett-Packard, Wilmington, DE) consisting of a G1312A binary pump, a G1329A automatic sample injector, and a G1315A diode array detector was used to perform HPLC analysis. Sample Preparation. Approximately 0.20 g powdered ginseng was accurately weighed into a 50 mL conical flask, and 10 mL 70% methanol was added. The suspension was sonicated for 30 min, and the sample solution was filtered through a 0.45 μm filter and used as the test solution for quantitative analysis of ginsenosides in Radix Ginseng. Chromatographic Conditions. HPLC analysis of Radix Ginseng was performed on an Alltima C18 HPLC column (4.6 mm × 250 mm, 5 μm) at 25°C with a sample injection volume of 20 μL. The mobile phase was a gradient elution of KH2PO4 buffer (2 mmol/L, pH 6.8) and acetonitrile, starting isocratically with 21% acetonitrile for 15 min and increasing to 38% acetonitrile over 55 min. The flow rate of the mobile phase was 1.0 mL/min, and the detector wavelength was 203 nm.
