Anti-Proliferative Effect of an Aqueous Extract of Prunella vulgaris in Vascular Smooth Muscle Cells
Figure 1
Effect of APV on high glucose-induced HASMCs proliferation, migration, and invasion. (a) Confluent HASMCs were incubated with or without APV and high glucose for 24 h and then treated with [3H]-thymidine for 24 h. (b) HASMCs were incubated approximately 80% confluent and then made a scratch with yellow tip. HASMCs were pretreated with APV for 1 h, which was followed by treatment with high glucose at 37°C for 24 h. ((A): wound, (B): control, (C): HG (25 mM), (D): HG + APV 0.1 μg/mL, (E): HG + APV 1 μg/mL, (F): HG + APV 10 μg/mL). (c) HASMCs on the 24-well invasion chambers were incubated with APV for 1 h, which was followed by treatment with high glucose at 37°C for 24 h. ((A): control, (B): HG (25 mM), (C): HG + APV 0.1 μg/mL, (D): HG + APV 1 μg/mL, (E): HG + APV 10 μg/mL). Values were expressed as mean ± S.E. of five independent experiments. (d) Each electrophoretogram is representative of the results from three individual experiments. ** versus control; ## versus high glucose alone.
