Anti-Proliferative Effect of an Aqueous Extract of Prunella vulgaris in Vascular Smooth Muscle Cells
Figure 3
Effects of APV on high glucose-induced MMP-2 or -9 activation and expression. (a) Conditioned supernatant was prepared, and the levels of MMP-2/-9 were measured by gelatin zymography, as described in Section 2. Electrophoretogram is representative of the results from three individual experiments. (b) The total cellular protein (40 μg) extracts were prepared and separated on 10% SDS-PAGE and blotted with the antibodies specific for MMP-2, MMP-9, and β-actin. Each electrophoretogram is representative of the results from three individual experiments. (c) The HASMCs were transiently transfected with pGL4.MMP-9-Luc2 and pCMV-β-gal. This was followed by harvesting, and their luciferase activities were determined. Values were expressed as mean ± S.E. of three individual experiments. ** versus control; # versus high glucose alone.
